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Synergistic response of pogostemon cablin (blanco) benth cell suspension cultures to precursor feeding and elicitation towards the productions of patchouli alcohol /Dr. Khairiah Abd Karim

Synergistic response of pogostemon cablin (blanco) benth cell suspension cultures to precursor feeding and elicitation towards the productions of patchouli alcohol

Abstract The individual and combined effects of precursor addition using cis-farnesol (CF) and elicitation using methyl jasmonate (MeJA), chitosan (CHI), yeast extract (YE) and Pycnoporous sanguineus (PS) were investigated to enhance the synthesis of patchouli alcohol (PA) in suspension cultures of Pogostomon cab/in (Blanco) Benth. Cultures added with CF and individual elicitation showed enhanced PA production up to 8 folds (267 mgL-1 in comparison to the control cultures (34.4 mgl- 1 The highest amount PA production (400 mg- l 1 was obtained in the cultures elicited with MeJA (100 1JM), while 1 cultures elicited with CHI (50 mg- l) showed a reduction of PA production after 5 days of elicitation. Meanwhile, the addition of YE ond PS showed negative results. The combination of CF and MeJA resulted in 20-fold incroment of PA production (610 mgl- 1 , showing the synergistic effect of these two production str 1tt•g1es

Contributor(s): Dr. Khairiah Abd Karim - Author Language: English Subject Keywords: Pogostomon ; patchouli ; chitosan First presented to the public: 24/7/2018 Original Publication Date: 3/21/2019 Previously Published By: Universiti Sains Malaysia Place Of Publication: School of Electrical & Electronic Engineering Citation: Extents: Number of Pages - 24 License Grantor / Date Granted:   / ( View License ) Date Deposited 2019-03-21 12:48:46.953 Date Last Updated 2019-03-21 12:50:46.877 Submitter: Nor Hayati Ismail

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