Proses untuk mengkaji kandungan asid ferulic (FA) dalam kulit buah M.oleifera telah dijalankan. Tujuan kajian ini adalah untuk mendapatkan optimum FA dalam keadaan optimum. Tiga faktor telah dikaji iaitu kepekatan NaOH, masa hidrolisis dan nisbah pepejal kepada pelarut. Selain itu, pembandingan antara asid hidrolisis dan alkali hidrolisis telah dijalankan untuk mengetahui yang mana paling sesuai untuk mengeluarkan kandungan FA optimum dan dapati bahawa alkali hidrolisis lebih sesuai. Komponen-komponen bioaktif telah dikenal pasti dengan menggunakan GC-MS iaitu brucine N-oxide, brucine, methyl cholate, fluocinonide, bianthrone, feulic acid, cholestan-6-one, pirenzepine, quinine, lexacalcitol, cymarin, isodrin, hydrocortisone, spironolactone dan naltrexone. Keadaan optimum adalah beikut: 3 jam masa hidrolisis, 1.25M kepekatan NaOH dan 1:10 nisbah pepejal kepada pelarut.
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The process of M.oleifera seed pods alkaline hydrolysis to investigate the amount of ferulic acid (FA) content was investigated. The purpose of this work was to obtain optimum FA content in the extract at optimum extraction condition. The three variables investigated were NaOH concentration, hydrolysis time and solid to solvent ratio. Besides, the effectiveness of acidic and alkaline hydrolysis on M.oleifera was found that alkaline hydrolysis was more suitable for FA extraction from M.oleifera. The major bioactive compounds were found using GC-MS identification which are brucine N-oxide, brucine, methyl cholate, fluocinonide, bianthrone, feulic acid, cholestan-6-one, pirenzepine, quinine, lexacalcitol, cymarin, isodrin, hydrocortisone, spironolactone and naltrexone. The optimum condition for FA extraction were: 3 hours hydrolysis time, 1.25M NaOH concentration and 1:10 solid to solvent ratio.