Aplikasi enzim di dalam industri sentiasa meningkat dari semasa ke semasa. Dalam usaha untuk meningkatkan aplikasi enzim, terdapat banyak kajian telah diperkenalkan. Lipase dari Candida Rugosa telah diimobilisasi di dalam butiran alginat hidrogel untuk aplikasi yang sesuai. Enzim yang terperangkap semasa titikan campuran algid an enzim di dalam cecair pengeras, kalsium ion. Kesan kepekatan alginat enzim lipase, suhu di keadaan optimum dan tahap boleh guna semula telah di kaji. Semakin meningkat kepekatan alginat, semakin meningkat unit aktiviti. Hasil immobilisasi (%) tertinggi adalah 99.17% dan unit aktiviti tertinggi adalah 2.365 IU/mg. Di bawah keadaan optimum dari analisis Design of Experiment (DoE), 0.03 g/ml kepekatan lipase dan 2% kepekatan alginat dan tahap guna semula enzim telah menyumbang kepada unit aktiviti 70.28%. Manakala, unit aktiviti untuk enzim teleh meningkat apabila suhu semasa rendam menaik. Suhu optimum untuk enzim adalah 37oC yang telah menyumbang kepada unit aktiviti yg tinggi. (0.331 IU/mg). Namun begitu, unit aktiviti bagi enzim telah menurun apabila suhu mencecah kepada 400C hingga 55oC. Keadaan ini berkemungkinan bahawa enzim telah pecah semasa suhu tinggi yang menyumbang kepada unit aktiviti yang rendah (0.037 IU/mg).
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Applications of enzyme in industry are becoming more relevant these days. In an effort to increase the application of enzyme, several techniques have been introduced in the research. In the present work, lipase from Candida rugosa was immobilized on alginate beads for possible applications. The enzyme was entrapped by drop-wise addition of an aqueous mixture of sodium alginate and the biocatalyst to the hardening solution of calcium ion. Effect of alginate concentration, lipase concentration, temperature at optimum conditions and reusability of the enzyme were investigated. An increased in alginate concentration give higher unit activity of the enzyme. This techniques provided gives higher immobilization yield (%) and activity yield at 99.17% and 2.365 IU/mg respectively. Under optimal conditions generate by ANOVA analysis using Design of Experiment (DoE) software ( 0.03 g/ml of lipase concentration and 2% of alginate concentration), the lipase beads retained enzyme activity equivalent to 70.28%. The optimum temperature suitable for immobilized enzyme also studied. Enzyme activity increases when the temperature was increased to 37oC, then decreases at higher temperature. The optimum temperature was determined at 37oC with unit activity of 0.331 IU/mg. At temperature 55oC, the activity declined to 0.037 IU/mg which suggested due to denaturation of lipase structure.
Immobilization of lipase from candida rugosa using alginate bead / Nursyarinie Nabihah Azeman