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Synergistic response of pogostemon cablin (blanco) benth cell suspension cultures to precursor feeding and elicitation / Dr.Derek Chan Juinn Chieh, Fatin Izzatie Mazlan

Synergistic response of pogostemon cablin (blanco) benth cell suspension cultures to precursor feeding and elicitation_Dr.Derek Chan Juinn Chieh, Fatin Izzatie Mazlan
This research investigates the synergistic effect of precursor feeding and elicitation towards the production of patchouli alcohol (PA) from Pogostemon cab/in callus and cell suspensron cultures rn shake flask systems. Initial study on feeding cis-farnesol as the precursor to activate PA production revelead tho prt sence of 68 mg/ml of PAin the culture system. Screening of abiotic and biotic elicitors was carried out to find the best elicitor that is able to enhance PA production. Out of four different elicitors from both categories, around 984 mg/L ot PA was produced when elicited with methyl jasmonate, MeJA (50 JlM concentration) after 3 days of eliartation, <;hewing that MeJA could stimulate metabolic pathway leading to PA production. Elicitation using yeast extroct has also shown some production of PA at varying concentrations, but the addition of chitosan and Pycnoporus sangumeu, extract did not improve PA production. It was found that the combination of cis-farnesol feeding and MeJA elicitatron drd not further increase PA production, indicating that only the individually elicited cell suspension cultures of P. coblm is c:lpable in improving the PA yield.
Contributor(s):
Dr.Derek Chan Juinn Chieh - Author
Fatin Izzatie Mazlan - Author
Language:
English
Subject Keywords:
patchouli; alcohol; abiotic
Originally created:
7/24/2018
Original Publication Date:
12/30/2019
Previously Published By:
Universiti Sains Malaysia
Place Of Publication:
School of Chemical Engineering
Citation:
Extents:
Number of Pages - 24
License Grantor / Date Granted:
  / ( View License )
Date Deposited
2019-12-30 11:28:50.784
Submitter:
Nor Hayati Ismail

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Synergistic response of pogostemon cablin (blanco) benth cell suspension cultures to precursor feeding and elicitation / Dr.Derek Chan Juinn Chieh, Fatin Izzatie Mazlan1 2019-12-30 11:28:50.784
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