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The role of flocculant onto the flocculation and sedimentation of microalgae

The role of flocculant onto the flocculation and sedimentation of microalgae / Nurfarah Farhana Azenan
Biodiesel yang lebih mesra alam dan mampan menjadi sumber tenaga yang menarik bagi menggantikan bahan bakar fosil disebabkan krisis tenaga pada masa kini. Mikroalga didapati sebagai sumber yang menjanjikan penghasilkan biofuel dengan kaedah pengelompokan yang berkos rendah. Peranan pengelompok terhadap pelaksanaan kaedah pengelompokan dan pemendapan sel mikroalga telah dikaji dalam kajian ini. Pertama sekali, hasil biojisim, jumlah lipid, dan kestabilan sel Chlorella sp ditentukan mengikut hari pengkulturan yang berlainan (10-26 hari). Kemudian pada hari penuaian yang optimum, dos optimum (5-200 mg/L) kitosan dan poli(diallildimetilammonium klorida)(PDDA) yang diperlukan untuk mencapai kadar pemisahan sel yang maksimum telah dibandingkan. Selepas itu pada dos pengelompok yang optimum, kesan pH (pH 5-11) ke atas kadar pemendapan kendiri Chlorella sp. dan kesannya terhadap kadar pemendapan sel selepas dicampur dengan kitosan dan PDDA telah dikaji. Akhir sekali, prestasi keseluruhan kedua-dua pengelompok dibandingkan. Daripada keputusan, hasil biojisim yang optimum, jumlah lipid yang paling tinggi, dan kestabilan sel Chlorella sp. yang sekata ditemui pada hari penuaian ke-10. Kitosan telah didapati sebagai pengelompok yang paling berkesan untuk penuaian mikroalga dengan dos optimum sebanyak 30 mg/L untuk mencapai peratus pemisahan sel sebanyak 96.7 ± 0.7%, yang membentuk gumpalan sel bersaiz lebih besar yang memperolehi kadar pemendapan sel-sel mikroalga yang lebih cepat berbanding PDDA. Kadar pemendapan tertinggi didapati pada pH 7 hingga 9 bagi medium yang dicampur dengan kitosan dan pada pH 11 bagi medium yang dicampur dengan PDDA, manakala pH medium tidak memberi kesan yang ketara kepada kadar pemendapan kendiri sel mikroalga Chlorella sp. Kitosan telah didapati sebagai pengelompok yang efektif dalam menjerap dan mengelompok sel-sel mikroalga dalam medium kultur. _______________________________________________________________________________________________________ Biodiesel which is more environmentally friendly and sustainable is becoming an attractive energy resource in replacement for fossil fuel despite the energy crisis nowadays. Microalgae are recognized as a promising resource to produce biofuel by a low-cost flocculation method. The role of flocculant towards the performance of flocculation and sedimentation of microalgae cells are investigated in this study. Firstly, the yield of biomass, total lipid, and the cell stability of Chlorella sp. are determined with respect to culturing day (day 10-26). Then at optimum harvesting day, the optimum dosage (5-200 mg/L) of chitosan and poly(diallyldimethylammonium chloride)(PDDA) needed to achieve the maximum cell separation efficiency are compared. After that at optimum dosage of flocculants, the effect of pH (pH 5-11) toward the rate of self-sedimentation of Chlorella sp. and its effect on the settling rate of cells after mixed with chitosan and PDDA are studied. Lastly, the overall performance of both flocculant is compared. From the results, the optimum yield of biomass, the highest total lipid, and constant cell stability of Chlorella sp. was found at day 10th of cultivation. Chitosan was found to be an effective flocculant for harvesting microalgae with optimum dosage of 30 mg/L to achieve cell separation efficiency of 96.7 0.7%, which form larger floc size which derive the faster sedimentation rate of microalgae cells compared to PDDA. Highest sedimentation rate was found in pH 7 to 9 for chitosan-added medium and at pH 11 for PDDA-added medium, while pH of medium does not significantly affect the rate of self-sedimentation of Chlorella sp. microalgae cells. Chitosan was found to be an effective flocculant in terms of adsorbing and coagulating the microalgae cells in the culture medium.
Contributor(s):
Nurfarah Farhana Azenan - Author
Primary Item Type:
Final Year Project
Identifiers:
Accession Number : 875005626
Language:
English
Subject Keywords:
Biodiesel; replacement; Microalgae
First presented to the public:
6/1/2015
Original Publication Date:
8/4/2020
Previously Published By:
Universiti Sains Malaysia
Place Of Publication:
School of Chemical Engineering
Citation:
Extents:
Number of Pages - 86
License Grantor / Date Granted:
  / ( View License )
Date Deposited
2020-08-04 12:10:50.169
Submitter:
Mohd Jasnizam Mohd Salleh

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