BS 6068-5.17:1995 Water quality. Part 5. Biological methods - Section 5.17 Determination of the prolonged toxicity of substances to freshwater fish. Method for evaluating the effects of substances on the growth rate of rainbow trout [Oncorhynchus mykiss Walbaum (Teleostei, Salmonidae)]
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This International Standard specifies a method for the
determination of the long-term sublethal toxicity of
substances soluble in water under specified con-
ditions to a species of anadromous fish
[Oncorhynchus mykiss Walbaum (Teleostei, Salmo-
nidae) common name, rainbow trout] in fresh
water.
NOTE 1 "Substances" is considered to include pure.
chemicals tested singly or in mixtures, waste waters, pro-
cess waters or other waters whose sublethal toxicity to fish
it is necessary to determine. A range of concentrations of
waste and process waters should be tested (see 8.2).
The response measured is the change in rate of
growth of fish exposed to the test substance over
periods of 14 d and 28 d (see [1] in annex B).
NOTE 2 The mass of each fish at the start of the test, the
period of exposure, the feeding regime and temperature
range have been chosen to ensure that the control fish will
be growing exponentially throughout the test.
The method is applicable for assigning, for each test
substance, a category of prolonged sublethal toxicity
to Oncorhynchus mykiss under the test conditions.
The results are insufficient by themselves to define
water quality limits for environmental protection.
It is possible for the method to be adapted for use
with other freshwater fish and marine and brackish
water fish with appropriate modification of the test
conditions, particularly with respect to the tempera-
ture, the quantity and quality of the dilution water, the
food and the fish-marking technique.
BS 6068-5.17:1995 Water quality. Part 5. Biological methods - Section 5.17 Determination of the prolonged toxicity of substances to freshwater fish. Method for evaluating the effects of substances on the growth rate of rainbow trout [Oncorhynchus mykiss Walbaum (Teleostei, Salmonidae)]
