Nanopartikel perak boleh disintesis menggunakan kaedah fizikal, kimia dan biologi. Antara tiga kaedah ini, pendekatan biologikal lebih mudah, mesra persekitaran dan berkos rendah. Kajian ini memberi tumpuan kepada biosintesis nanopartikel perak menggunakan ekstrak daun ubi kayu sebagai agen penurunan dan penstabilan. Apabila ion perak akueus terdedah kepada ekstrak daun, ia akan diturunkan dan menghasilkan perubahan warna menandakan pembentukan nanopartikel perak. Partikel perak yang sudah disintesis akan disifatkan dengan menggunakan teknik- teknik Spektroskopi UV-Vis, Mikroskop Elektron Pengimbas, Penyerakan Cahaya Dinamik, Penyebaran Tenaga X-ray dan Analisis Keupayaan Zeta. Spektrum UV-Vis yang dihasilkan oleh sampel mengandungi nanopartikel perak menunjukkan peningkatan secara beransuransur
pada 430 nm hingga 435 nm. Antara parameter yang dikaji adalah penggunaan kepekatan ekstrak daun ubi kayu yang berbeza, nisbah isipadu ekstrak tumbuhan kepada kepekatan argentum nitrat. Kajian tersebut juga menunjukkan julat jarak
gelombang yang sama pada penyerapan maksimum. Serapan tertinggi, 2.00 didapati selepas 3 hari menggunakan 2 mM argentum nitrat bertindak balas dengan 1% ekstrak tumbuhan dalam ratio 1:5. Analisis Keupayaan Zeta memberikan nilai -15.8 mV. Purata saiz bagi nanopartikel perak adalah di antara julat 12 nm hingga 1000 nm.
_______________________________________________________________________________________________________
Silver nanoparticles can be synthesized using physical, chemical and biological pathway. As compared to these three methods, biological approach is easier, more environmentally friendly and low cost. This study emphases on biosynthesis of silver nanoparticles using Cassava leaf extract as reducing and stabilizing agents. Aqueous silver ions, Ag+ when exposed to plant leaf extract were reduced to silver metal, Ag and resulted in a colour change from colourless to brown indicating the formation of silver nanoparticles. The synthesized silver nanoparticles were characterized by UVVis Spectroscopy, Surface Electron Microscopy (SEM), Energy Dispersive X-ray Spectroscopy (EDX), Dynamic Light Scattering (DLS) and Zeta Potential Analysis techniques. The UV-vis spectrum of sample containing synthesized silver showed gradual increase of surface plasmon resonance peak intensity at 430 nm to 435 nm. A few parameters such as plant extract concentration, volume ratio of plant extract to concentration of silver nitrate and silver nitrate concentration have been studied and show the same range of wavelength at maximum absorption. The highest absorbance, 2.00 was obtained after 3 days using 2 mM silver nitrate react with 1 wt% plant extract in ratio of 1:5. Zeta Potential Analysis for silver nanoparticles’ stability shows the
value of -15.8 mV. The hydrodynamic size distribution was analyzed using DLS which gives average Z-diameter values at the range between 12 nm to 1000 nm.