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Magnetophoretic separation of protein by surface functionalized nanoparticles with low gradient magnetic separation

Magnetophoretic separation of protein by surface functionalized nanoparticles with low gradient magnetic separation / Chai Chuan Chun
Dalam kertas kerja ini, nanopartikel besi oksida yang difungsikan dengan PDDA yang pelbagai saiz digunakan untuk menjalankan pemisahan protein. PDDA yang bersaiz sederhana memberikan keputusan yang terbaik dalam kajian ini. Dengan interaksi electrosterik, 500ppm nanopartikel besi oksida mampu memisahkan sebanyak 408.18ppm protein. Tindak balas nanopartikel besi oksida terhadap magnet telah membolehkan nanopartikel mudah dikumpul dengan menggunakan magnet kekal. Selain itu, untuk applikasi nanopartikel dalam badan manusia, nanopartikel besi oksida telah difungsikan dengan pluronic f-127 untuk menghalang penjerapan protein kepada permukaan nanopartikel. Ini disubabkan protein mampunyai kepekatan yang sangat tinggi dalam darah manusia. Dalam kajian ini, nanopartikel yang difungsikan dengan pluronic f-127 mampu menghalang penjerapan protein kepada nanopartikel dengan sempurna. _______________________________________________________________________________________________________ Iron oxide nanoparticles coated with various size of PDDA are used to perform protein adsorption in this paper. Nanoparticles coated with medium size PDDA perform the best in this study. With electrostatic attraction, 500ppm of coated nanoparticle was able to adsorb as much as 408.18ppm of protein. The magnetic responsive behavior of iron oxide nanoparticles enables its easy collection with only a permanent magnet. Besides, for the application of iron oxide nanoparticles to human body, pluronic f-127 was used to functionalize the iron oxide nanoparticles to prevent protein adsorption as protein is at high concentration in human blood. From the study, it shows that nanoparticle was able to prevent the adsorption of protein perfectly with pluronic f-127 coating on it.
Contributor(s):
Chai Chuan Chun - Author
Primary Item Type:
Final Year Project
Identifiers:
Accession Number : 875005169
Language:
English
Subject Keywords:
nanoparticles; PDDA; adsorption
First presented to the public:
6/1/2013
Original Publication Date:
1/27/2020
Previously Published By:
Universiti Sains Malaysia
Place Of Publication:
School of Chemical Engineering
Citation:
Extents:
Number of Pages - 53
License Grantor / Date Granted:
  / ( View License )
Date Deposited
2020-01-28 16:26:42.55
Submitter:
Mohd Jasnizam Mohd Salleh

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