Sebuah reaktor bersesekat anaerob terubahsuai telah digunakan untuk merawat efluen kilang kertas kitar semula pada empat masa tahanan hidraulik, (HRT) 1, 3, 5 dan 7 hari. Tahap kecekapan kadar buangan permintaan oksigen kimia (COD) adalah masing-masing pada 78.37%, 97.21%, 96.84% dan 94.53%. Di dalam kajian ini, model matematik seperti Monod, Grau peringkat kedua dan model Stover-Kincannon terubahsuai telah digunakan untuk menentukan kinetik penyingkiran substrat oleh reaktor bersesekat anaerob terubahsuai. Parameter kinetik telah dikenalpasti melalui linear regresi dengan menggunakan data eksperimen. Didapati bahawa data simulasi mempunyai hubungan yang baik dengan model matematik yang telah digunakan. Di samping itu, pekali penentuan, (R2) yang diperolehi daripada nilai eksperimen dan ramalan juga mengesahkan kesesuaian model kinetik. Aktiviti pelbagai jenis mikroorganisma dalam reaktor adalah salah satu faktor yang telah mempengaruhi pencernaan anaerobik yang akan menghasilkan metana. Mikroskop Imbasan Elektron (SEM) dan pendarfluor mikroskop telah digunakan untuk memerhatikan mikroorganisma anaerobik yang terlibat di dalam reaktor semasa penghadaman anaerobik. Daripada eksperimen, didapati bahawa bakteria yang bertanggungjawab ke atas biodegradasi biomas dalam reaktor bersesekat anaerob terubahsuai adalah lebih dominan. Di samping itu, bakteria yang berkembang pesat adalah bakteria yang mampu untuk berkembang di bawah tahap substrat yang tinggi dan pH yang lebih rendah adalah lebih dominan di petak hadapan reactor. Manakala bakteria yang berkembang lebih baik pada pH yang lebih tinggi didapati pada petak hujung reaktor. Di samping itu, jumlah Methanosarcina dan Methanosaeta adalah lebih tinggi berbanding dengan bakteria lain yang menghasilkan metana di dalam reaktor.
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A laboratory-scale modified anaerobic baffled reactor was used to treat recycled paper mill effluent at four different hydraulic retention times (HRTs) of 1, 3, 5 and 7 days. The COD removal efficiency of the reactor were 78.37%, 97.21%, 96.84% and 94.53%, respectively. In this study, mathematical model such as Monod, Grau second order and modified Stover-Kincannon models were applied to determine the substrate removal kinetic in modified anaerobic baffled reactor. The kinetic parameters were determined from linear regression using experimental data. It was found that the simulated data were in good agreement with the mathematical models that were being used. In addition, the coefficient of determination (R2) obtained from the experimental and predicted value also confirmed the suitability of the kinetic models. Activities of various kind of microorganism in the reactor were one of the factors that affected the anaerobic digestion that will produce methane. Scanning electron microscope (SCE) and fluorescence microscope were used to observe the anaerobic microorganism involved in the reactor during anaerobic digestion. From the experiment, it was found that the bacteria responsible biodegradation of biomass in the MABR were dominant. In addition, it was observed that fast growing bacteria which are capable to grow under high substrate level and lower pH were dominant at front compartment of the reactor. In the meantime, slow growing scavenging bacteria which grow better at higher pH were found at the end of the reactor. In addition, population of Methanosarcina and Methanosaeta might be higher compared to the other available methane former bacteria in the reactor.
Kinetic and fluorescence studies of recycled paper mill effluent treatment using modified anaerobic baffled reactor / Mohd Khairi Othman