Diatom telah menjadi minat utama bagi kajian dalam pengeluaran eicosapentanoic asid EPA kerana kebanyakan diatom mengandungi kandungan EPA yang tinggi. Dalam kajian ini, eksperimen telah dijalankan untuk mengkaji kesan sumber nitrogen and fosfat terhadap pertumbuhan dan pengeluaran EPA oleh Amphora sp. Kesan daripada sumber nitrogen telah dikaji dengan menggunakan kepekatan yang berbeza KNO3 diantara 0.066 g/L ke 0.265 g/L dengan kepekatan larutan stok pada 216g / L. Untuk sumber fosfat, eksperimen telah dijalankan dengan menggunakan kepekatan natrium fosfat dihydrogen (NaH2PO4.H2O) yang berbeza diantara 0.007g/L ke 0.027 g/L dengan kepekatan larutan stok pada 20g / L. Dalam kajian ini, pengeluaran biojisim dan kandungan EPA telah dipantau. Jumlah tertinggi biomas diperolehi pada 0.066 g/L iaitu 137.6 5.33 mg / L dan 0.013 g/L pada 83.33 10.09 mg / L. Daripada kajian ini, keputusan menunjukkan bahawa hasil EPA adalah berkadar terus dengan jumlah biojisim yang dihasilkan. Walau bagaimanapun, terdapat adalah satu penyelewengan dalam keputusan seperti sumber kepekatan nitrogen rendah memberikan pertumbuhan yang lebih baik untuk Amphora sp. Keputusan yang diperolehi menunjukkan bahawa faktor pemakanan seperti nitrogen dan fosfat menunjukan akan memberi kesan kepada pertumbuhan dan komposisi biokimia Amphora sp.
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Diatoms have become major interest for study in the production of eicosapentanoic acid EPA as most diatoms contain high content of EPA. In the present study, experiments were conducted in triplicates to investigate the effect of nitrogen source and phosphate source towards Amphora sp. growth and EPA production. The effect of nitrogen source was studied by using different concentration of KNO3 between 0.066 g/L to 0.265 g/L with the stock solution concentration at 216g/L. For phosphate source, the experiment were conducted by using different concentration of sodium dihydrogen phosphate (NaH2PO4.H2O) between 0.007g/L to 0.027 g/L with stock solution concentration at 20g/L. In this research, the biomass production and EPA content were monitored. Highest amount of biomass were obtained at 0.066 g/L which was 137.6 + 5.33 mg/L and P1.0mL/L at 83.33 + 10.09 mg/L. The production of EPA was highest when the cells were cultivated at 0.066 g/L nitrate concentration. From this study, the results showed that the EPA yield is directly proportional to the amount of biomass produced. However, the there is an irregularities in the results as the less nitrogen source concentration gives a better growth to Amphora sp. The result obtained implies that nutritional factors such as nitrogen and phosphate.
Effects of nitrate and phosphate on optimization of eicosapentanoic acid (epa) and growth rate by amphora sp / Muhammad Hafiz Wan