Sistem Anammox mengandungi ammonium dan nitrit, sebagai satu-satunya penderma elektron dan penerima elektron, manakala karbonat merupakan satu-satunya sumber karbon yang disediakan. Kehadiran nitrit dan ketiadaan penderma elektron organik adalah penting untuk aktiviti Anammox. Kajian kebuluran telah dijalankan kerana kebuluran bakteria adalah fenomena yang biasa di alam dan dalam proses rawatan air buangan, terutama di bawah keadaan persekitaran yang bervariasi dan rumit dalam biofilem. Tiga jenis kebuluran diperhatikan semasa kajian ini, iaitu kelaparan nitrit, kelaparan amonia dan kelaparan kedua-dua nitrit dan amonia dengan penambahan hidrazin. Jenis kebuluran ini disiasat dengan tempoh masa kebuluran yang sama selama 15 hari pada suhu operasi 35 ˚C. Semasa tempoh kebuluran, kepekatan komponen nitrogen (ammonia dan nitrit) di dalam air sisa diperhatikan untuk mengkaji kesan kebuluran pada proses Anammox. Sementara, Cecair Bercampur Campur Pepejal (MLSS), Cecair Becampur Campir Pepejal Meruap (MLVSS) dan bahan polimer tambahan (EPS) bakteria anammox diperhatikan semasa tempoh kebuluran untuk mengkaji kesan kebuluran bakteria. Telah didapati bahawa, kebuluran nitrit dalam proses Anammox memberikan sedikit penyingkiran kepekatan
ammonia dalam air sisa yang dari 50 mg/L hingga 33.8 mg/L selepas tempoh kebuluran. Seterusnya, kebuluran ammonia dan kebuluran kesuluruhan dengan penambahan hidrazin masing-masing menghasilkan penghapusan nitrit dan hidrazin.
Jumlah kelaparan dengan penambahan hydrazine memberikan kepekatan tertinggi MLSS dan MLSS, iaitu 250 mg / L dan 170 mg / L masing-masing.
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Anammox system contained ammonium and nitrite, as the only electron donor and electron acceptor, respectively, while carbonate was the only carbon source provided. The presence of nitrite and the absence of organic electron donors are
essential for Anammox activity. Starvation studies have been carried out because bacterial starvation is a common phenomenon in nature and in wastewater treatment process, especially under conditions of varying influent and complicated environment in the biofilm. Three types of starvation were observed during this study, which is starvation of nitrite, starvation of ammonia and starvation of both nitrite and ammonia with addition of hydrazine. These types of starvation were investigated with same period of starvation time of 15 days at operating temperature of 35 ˚C. During the starvation period, the concentration of nitrogen component (ammonia and nitrite) in the wastewater were observed to study the effects of starvation to the Anammox process. While, Mixed Liquor Suspended Solid (MLSS), Mixed Liquor Volatile Suspended Solid (MLVSS) and extra polymeric substances (EPS) of the anammox bacteria was observed during the starvation period to study the effects of starvation to the bacteria. It have been found that, starvation of nitrite in the Anammox process gives small removal of ammonia concentration in the wastewater which is from 50 mg/L to 33.8 mg/L after the starvation period. While, starvation of ammonia and total starvation with addition of hydrazine result in total removal of nitrite and hydrazine respectively. Total starvation with addition of hydrazine give the highest concentration of MLSS and MLSS, which is 250 mg/L and 170 mg/L respectively.
Effects of starvation on anammox process and bacteria / Aidil Faizie Shadan_