Penghibridanan organik/organic biasanya digunakan untuk mensintesis bahan berfungsi. Walaubagaimanapun, ia jarang dipertimbangkan dalam rawatan air sisa industri. Melalui sekat gerak anionik (mis Acid Violet 7, Direct Blue 71) - kationik (mis Brilliant Green) pewarna kompleks dengan kalsium karbonat yang berkembang, pewarna konjugat penghibridan telah dibentangkan. Morfologi 3D dan struktur hybrid konjugat dicirikan dengan menggunakan Brunauer-Emmett-Teller (BET), jelmaan Fourier spektroskopi inframerah (FTIR) dan Analisis Elemental dan mekanisme pembentukan dijelaskan. Satu langkah penghibridan yang mudah digunakan dalam rawatan air sisa organic pekat. Teknik ini melibatkan penjerapan, pemberbukuan dan kompleksasi ion dan menyebabkan penyingkiran bahan-bahan organik pada tahap yang tinggi. Dengan "menggunakan sisa untuk merawat sisa", rawatan air sisa yang kos efektif dan mesra alam mudah dicapai. Teknik ini telah diuji dengan menggunakan air sisa industry dan air sungai. Penyingkiran pewarna paling baik telah diperolehi bagi penggunaan 1mM Acid Violet 7 untuk merawat 1mM Brilliant Green iaitu 97.53% manakala untuk merawat sampel sisa industri, penyingkiran pewarna yang paling tinggi 83% dengan 0.5 mM Direct Blue 71. Ini telah menjelaskan ion pasangan keseimbangan antara pewarna anionik dan pewarna kationik.
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Inorganic/organic hybridization is usually used to synthesize functional materials. However, it is rarely considered in industrial wastewater treatment. By immobilizing the anionic (e.g. Acid Violet 7, Direct Blue 71)–cationic (e.g. Brilliant Green) dye complex with growing calcium carbonate, dye conjugate hybridization was presented. The structure of the conjugate hybrid were characterized by using Brunauer–Emmett–Teller (BET), Fourier transform infrared spectroscopy (FTIR), spectrophotometry and elemental analysis and the mechanism of formation was explained. The simple single-step hybridization applied in treatment of concentrated organic wastewaters. This technique involves adsorption, flocculation and ionic complexation and resulted a high level of removal of organic substances. By “using waste to treat waste”, a convenient, cost-effective and environment-friendly wastewater treatment was accomplished. This technique was tested by using industrial wastewater and river water. The best dye removal was obtained for the use of 1mM of Acid Violet 7 to treat 1mM of Brilliant Green which is 97.53% whereas for treating industrial waste sample, the highest dye removal of 83% with the 0.5mM of Direct Blue 71. This explained ion pair equilibrium between the anionic dye and cationic dye.