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The effect of growing cells and in-situ biotransformation of 2,6,6-trimethylcyclohex-2-ene-1,4-dione towards products formation

The effect of growing cells and in-situ biotransformation of 2,6,6-trimethylcyclohex-2-ene-1,4-dione towards products formation / Siva Sree Hari Muniandy
Actinol adalah substrat yang diperlukan untuk sintesis xanthophylls optik aktif, xanthoxin dan zeaxanthins serta aroma konstituen tembakau dan kunyit boleh dihasilkan oleh pengurangan ketoisophorone dengan hasil yang tinggi dan enantioselectivity. Actinol boleh dihasilkan dari biotransformasi ketoisophorone. Biotransformasi daripada ketoisophorone membentuk levodione, yang merupakan perantaraan sebelum menghasilkan actinol.Biotransformasi daripada ketoisophorone telah dikaji semasa fasa pertumbuhan S. cerevisiae dengan penggunaan dan tanpa glukosa. Semasa fasa pertumbuhan S. cerevisiae, biotransformasi ketoisophorone hanya mampu menghasilkan pertengahan dan produk itu belum terbentuk.Kadar biotransformasi daripada ketoisophorone adalah lebih cepat dengan penggunaan glukosa. Hal ini kerana kehadiran glukosa meningkatkan konsentrasi NADH dan NADPH yang merupakan kofaktor penting yang diperlukan untuk biotransformasi daripada ketoisophorone. Oleh itu, kadar pengurangan ketoisophorone adalah lebih tinggi menyebabkan konsentrasi yang lebih tinggi levodione. Apabila kadar biotransformasi adalah tinggi, pengeluaran actinol juga akan lebih cepat. Oleh itu, kehadiran glukosa adalah penting bagi biotransformasi ketoisophorone. _______________________________________________________________________________________________________ Actinol (ACT) is the substrate needed for the synthesis of optically active xanthophylls, xanthoxin and zeaxanthins as well as aroma constituent of tobacco and saffron can be produced by the reduction of ketoisophorone (KIP) with high yield and enantioselectivity. The ACT can be produced from the biotransformation of KIP. The biotransformation of KIP forms levodione (DOIP), which is intermediate before further reduction into ACT. The biotransformation of KIP was studied during the growth phase of S. cerevisiae with the presence and absence of glucose. During the growth phase of S. cerevisiae, the biotransformation of KIP was only able to produce intermediate and the product was yet to be formed. The rate of biotransformation of KIP was faster with the presence of glucose. This was because presence of glucose increases the concentration of NADH and NADPH which was the essential cofactor needed for biotransformation of KIP. Thus, the rate of reduction of KIP was higher resulting in higher concentration of DOIP. When the rate of biotransformation was high, the production of ACT will also be faster. Therefore, the presence of glucose was important for the biotransformation of KIP.
Contributor(s):
Siva Sree Hari Muniandy - Author
Primary Item Type:
Final Year Project
Identifiers:
Barcode : 00003107194
Accession Number : 875007314
Language:
English
Subject Keywords:
Actinol (ACT); biotransformation of KIP; presence of glucose
First presented to the public:
5/1/2017
Original Publication Date:
4/30/2018
Previously Published By:
Universiti Sains Malaysia
Place Of Publication:
School of Chemical Engineering
Citation:
Extents:
Number of Pages - 45
License Grantor / Date Granted:
  / ( View License )
Date Deposited
2018-04-30 12:17:16.384
Date Last Updated
2019-01-07 11:24:32.9118
Submitter:
Mohd Jasnizam Mohd Salleh

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The effect of growing cells and in-situ biotransformation of 2,6,6-trimethylcyclohex-2-ene-1,4-dione towards products formation1 2018-04-30 12:17:16.384