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Deposition of nylon membrane on carbon electrode for the development of ascorbic acid electrochemical biosensor

Deposition of nylon membrane on carbon electrode for the development of ascorbic acid electrochemical biosensor / Noor Nadhirah Harith
Biosensor elektrokimia adalah gabungan kaedah elektroanalitikal dengan kadar sensitif yang tinggi kerana kehadiran sistem pengesan sejenis biomolekul. Tujuan utama penyelidikan ini dilaksanakan adalah untuk mengkaji penyatuan antara asid askorbik (AA) dengan polyaniline (PANI) dan untuk menyiasat jenis - jenis respons elektrokimia yang terdiri daripada kitaran voltametri dan galangan elektrokimia spektroskopi. Dalam penyelidikan ini, penyatuan antara AA dan PANI dilakukan dengan mengsintesis PANI/Fe2O3 sebelum menggabungkannya dengan AA. Spektroskopi jenis UV-Vis digunakan untuk menyukat penyerapan untuk cecair berlainan iaitu PBS, 50 mM AA, PANI, PANI/Fe2O3 dan PANI/Fe2O3/AA dengan kepekatan 50 mM untuk memastikan penyatuan antara AA dan PANI/Fe2O3 berjaya. Seterusnya, jenis membrane-sensor disusun serta digabungkan iaitu, elektrod karbon-kaca, elektrod karbon-kaca bersama membrane serta elektrod karbon-kaca bersama membrane di mana, ascorbate oxidase dilekatkan di atas permukaan membrane tersebut. Respons kitaran voltametri dan galangan elektrokimia spektroskopi diukur untuk memerhati potensi pengoksidaan dan kekonduksian cecair tersebut _______________________________________________________________________________________________________ Electrochemical biosensor is the combination of electroanalytical methods of high sensitivity with a presence of biorecognition system that helps in identifying target species. The main objective of this work is to study the conjugation of ascorbic acid (AA) to polyaniline (PANI) and to explore the cyclic voltammetry and electrochemical impedance spectroscopy of the membrane-based sensor to detect AA. In this study, the conjugation of ascorbic AA was first done by the synthesis of PANI/Fe2O3, before the AA was interacted with PANI/Fe2O3. UV-Vis spectrometer absorbance for solutions PBS, AA of 50 mM, PANI, PANI/Fe2O3 and PANI/Fe2O3/AA of 50 mM were measured to prove the successful conjugation of AA. Next, membrane-sensor was assembled, bare glass carbon electrode (GCE), GCE-nylon membrane and GCE-nylon membrane immobilized with ascorbate oxidase were testing with AA. The cyclic voltammetry pattern and electrochemical impedance spectroscopy were recorded to study the redox potential and conductivity of the testing solutions.
Contributor(s):
Noor Nadhirah Harith - Author
Primary Item Type:
Final Year Project
Identifiers:
Accession Number : 875007551
Language:
English
Subject Keywords:
Electrochemical biosensor; electroanalytical methods; identifying target
First presented to the public:
6/1/2018
Original Publication Date:
7/18/2018
Previously Published By:
Universiti Sains Malaysia
Place Of Publication:
School of Chemical Engineering
Citation:
Extents:
Number of Pages - 86
License Grantor / Date Granted:
  / ( View License )
Date Deposited
2018-07-18 12:15:41.893
Date Last Updated
2019-01-07 11:24:32.9118
Submitter:
Mohd Jasnizam Mohd Salleh

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Deposition of nylon membrane on carbon electrode for the development of ascorbic acid electrochemical biosensor1 2018-07-18 12:15:41.893
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